LymphedemaV1, Main, Exploration, bibRecord, 001711

The indole alkaloid meleagrin, from the olive tree endophytic fungus Penicillium chrysogenum, as a novel lead for the control of c-Met-dependent breast cancer proliferation, migration and invasion

Identifieur interne : 001711 ( Main/Exploration ); précédent : 001710; suivant : 001712

The indole alkaloid meleagrin, from the olive tree endophytic fungus Penicillium chrysogenum, as a novel lead for the control of c-Met-dependent breast cancer proliferation, migration and invasion

Auteurs : Mohamed S. Mady ; Mohamed M. Mohyeldin ; Hassan Y. Ebrahim ; Heba E. Elsayed ; Wael E. Houssen [Royaume-Uni, Égypte] ; Eman G. Haggag ; Randa F. Soliman ; Khalid A. El Sayed

Source :

Bioorganic & medicinal chemistry [ 0968-0896 ] ; 2015.

RBID : PMC:4703546

Abstract

Fungi of the genus Penicillium produce unique and chemically diverse biologically active secondary metabolites, including indole alkaloids. The role of dysregulated hepatocyte growth factor (HGF) and its receptor, c-Met, in the development and progression of breast carcinoma is documented. The goal of this work is to explore the chemistry and bioactivity of the secondary metabolites of the endophytic Penicillium chrysogenum cultured from the leaf of the olive tree Olea europea, collected in its natural habitat in Egypt. This fungal extract showed good inhibitory activities against the proliferation and migration of several human breast cancer lines. The CH₂Cl₂ extract of P. chrysogenum mycelia was subjected to bioguided chromatographic separation to afford three known indole alkaloids; meleagrin (1), roquefortine C (2) and DHTD (3). Meleagrin inhibited the growth of the human breast cancer cell lines MDA-MB-231, MDA-468, BT-474, SK BR-3, MCF7 and MCF7-dox, while similar treatment doses were found to have no effect on the growth and viability of the non-tumorigenic human mammary epithelial cells MCF10A. Meleagrin also showed excellent ATP competitive c-Met inhibitory activity in Z-Lyte assay, which was further confirmed via molecular docking studies and Western blot analysis. In addition, meleagrin treatment caused a dose-dependent inhibition of HGF-induced cell migration, and invasion of breast cancer cell lines. Meleagrin treatment potently suppressed the invasive triple negative breast tumor cell growth in an orthotopic athymic nude mice model, promoting this unique natural product from hit to a lead rank. The indole alkaloid meleagrin is a novel lead c-Met inhibitory entity useful for the control of c-Met-dependent metastatic and invasive breast malignancies.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4703546

DOI: 10.1016/j.bmc.2015.11.038
PubMed: 26692349
PubMed Central: 4703546

Affiliations:

Royaume-Uni, Égypte

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The indole alkaloid meleagrin, from the olive tree endophytic fungus <italic>Penicillium chrysogenum</italic>
, as a novel lead for the control of c-Met-dependent breast cancer proliferation, migration and invasion</title>
<author><name sortKey="Mady, Mohamed S" sort="Mady, Mohamed S" uniqKey="Mady M" first="Mohamed S." last="Mady">Mohamed S. Mady</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="A2">Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Helwan, Cairo, Egypt, 11795</nlm:aff>
<wicri:noCountry code="subfield">11795</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Mohyeldin, Mohamed M" sort="Mohyeldin, Mohamed M" uniqKey="Mohyeldin M" first="Mohamed M." last="Mohyeldin">Mohamed M. Mohyeldin</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Ebrahim, Hassan Y" sort="Ebrahim, Hassan Y" uniqKey="Ebrahim H" first="Hassan Y." last="Ebrahim">Hassan Y. Ebrahim</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Elsayed, Heba E" sort="Elsayed, Heba E" uniqKey="Elsayed H" first="Heba E." last="Elsayed">Heba E. Elsayed</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Houssen, Wael E" sort="Houssen, Wael E" uniqKey="Houssen W" first="Wael E." last="Houssen">Wael E. Houssen</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland</wicri:regionArea>
<wicri:noRegion>Scotland</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A4">Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt</nlm:aff>
<country xml:lang="fr">Égypte</country>
<wicri:regionArea>Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516</wicri:regionArea>
<wicri:noRegion>Mansoura 35516</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Haggag, Eman G" sort="Haggag, Eman G" uniqKey="Haggag E" first="Eman G." last="Haggag">Eman G. Haggag</name>
<affiliation><nlm:aff id="A2">Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Helwan, Cairo, Egypt, 11795</nlm:aff>
<wicri:noCountry code="subfield">11795</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Soliman, Randa F" sort="Soliman, Randa F" uniqKey="Soliman R" first="Randa F." last="Soliman">Randa F. Soliman</name>
<affiliation><nlm:aff id="A2">Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Helwan, Cairo, Egypt, 11795</nlm:aff>
<wicri:noCountry code="subfield">11795</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="El Sayed, Khalid A" sort="El Sayed, Khalid A" uniqKey="El Sayed K" first="Khalid A." last="El Sayed">Khalid A. El Sayed</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">26692349</idno>
<idno type="pmc">4703546</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4703546</idno>
<idno type="RBID">PMC:4703546</idno>
<idno type="doi">10.1016/j.bmc.2015.11.038</idno>
<date when="2015">2015</date>
<idno type="wicri:Area/Pmc/Corpus">003966</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003966</idno>
<idno type="wicri:Area/Pmc/Curation">003965</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003965</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000D84</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000D84</idno>
<idno type="wicri:Area/Ncbi/Merge">007C54</idno>
<idno type="wicri:Area/Ncbi/Curation">007C54</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">007C54</idno>
<idno type="wicri:doubleKey">0968-0896:2015:Mady M:the:indole:alkaloid</idno>
<idno type="wicri:Area/Main/Merge">001713</idno>
<idno type="wicri:Area/Main/Curation">001711</idno>
<idno type="wicri:Area/Main/Exploration">001711</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The indole alkaloid meleagrin, from the olive tree endophytic fungus <italic>Penicillium chrysogenum</italic>
, as a novel lead for the control of c-Met-dependent breast cancer proliferation, migration and invasion</title>
<author><name sortKey="Mady, Mohamed S" sort="Mady, Mohamed S" uniqKey="Mady M" first="Mohamed S." last="Mady">Mohamed S. Mady</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="A2">Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Helwan, Cairo, Egypt, 11795</nlm:aff>
<wicri:noCountry code="subfield">11795</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Mohyeldin, Mohamed M" sort="Mohyeldin, Mohamed M" uniqKey="Mohyeldin M" first="Mohamed M." last="Mohyeldin">Mohamed M. Mohyeldin</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Ebrahim, Hassan Y" sort="Ebrahim, Hassan Y" uniqKey="Ebrahim H" first="Hassan Y." last="Ebrahim">Hassan Y. Ebrahim</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Elsayed, Heba E" sort="Elsayed, Heba E" uniqKey="Elsayed H" first="Heba E." last="Elsayed">Heba E. Elsayed</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Houssen, Wael E" sort="Houssen, Wael E" uniqKey="Houssen W" first="Wael E." last="Houssen">Wael E. Houssen</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland</wicri:regionArea>
<wicri:noRegion>Scotland</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A4">Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt</nlm:aff>
<country xml:lang="fr">Égypte</country>
<wicri:regionArea>Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516</wicri:regionArea>
<wicri:noRegion>Mansoura 35516</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Haggag, Eman G" sort="Haggag, Eman G" uniqKey="Haggag E" first="Eman G." last="Haggag">Eman G. Haggag</name>
<affiliation><nlm:aff id="A2">Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Helwan, Cairo, Egypt, 11795</nlm:aff>
<wicri:noCountry code="subfield">11795</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Soliman, Randa F" sort="Soliman, Randa F" uniqKey="Soliman R" first="Randa F." last="Soliman">Randa F. Soliman</name>
<affiliation><nlm:aff id="A2">Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Helwan, Cairo, Egypt, 11795</nlm:aff>
<wicri:noCountry code="subfield">11795</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="El Sayed, Khalid A" sort="El Sayed, Khalid A" uniqKey="El Sayed K" first="Khalid A." last="El Sayed">Khalid A. El Sayed</name>
<affiliation><nlm:aff id="A1">Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, Monroe, Louisiana, 71201</nlm:aff>
<wicri:noCountry code="subfield">71201</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Bioorganic & medicinal chemistry</title>
<idno type="ISSN">0968-0896</idno>
<idno type="eISSN">1464-3391</idno>
<imprint><date when="2015">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P2">Fungi of the genus <italic>Penicillium</italic>
 produce unique and chemically diverse biologically active secondary metabolites, including indole alkaloids. The role of dysregulated hepatocyte growth factor (HGF) and its receptor, c-Met, in the development and progression of breast carcinoma is documented. The goal of this work is to explore the chemistry and bioactivity of the secondary metabolites of the endophytic <italic>Penicillium chrysogenum</italic>
 cultured from the leaf of the olive tree <italic>Olea europea</italic>
, collected in its natural habitat in Egypt. This fungal extract showed good inhibitory activities against the proliferation and migration of several human breast cancer lines. The CH<sub>2</sub>
Cl<sub>2</sub>
 extract of <italic>P. chrysogenum</italic>
 mycelia was subjected to bioguided chromatographic separation to afford three known indole alkaloids; meleagrin (<bold>1</bold>
), roquefortine C (<bold>2</bold>
) and DHTD (<bold>3</bold>
). Meleagrin inhibited the growth of the human breast cancer cell lines MDA-MB-231, MDA-468, BT-474, SK BR-3, MCF7 and MCF7-dox, while similar treatment doses were found to have no effect on the growth and viability of the non-tumorigenic human mammary epithelial cells MCF10A. Meleagrin also showed excellent ATP competitive c-Met inhibitory activity in Z-Lyte assay, which was further confirmed via molecular docking studies and Western blot analysis. In addition, meleagrin treatment caused a dose-dependent inhibition of HGF-induced cell migration, and invasion of breast cancer cell lines. Meleagrin treatment potently suppressed the invasive triple negative breast tumor cell growth in an orthotopic athymic nude mice model, promoting this unique natural product from hit to a lead rank. The indole alkaloid meleagrin is a novel lead c-Met inhibitory entity useful for the control of c-Met-dependent metastatic and invasive breast malignancies.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Royaume-Uni</li>
<li>Égypte</li>
</country>
</list>
<tree><noCountry><name sortKey="Ebrahim, Hassan Y" sort="Ebrahim, Hassan Y" uniqKey="Ebrahim H" first="Hassan Y." last="Ebrahim">Hassan Y. Ebrahim</name>
<name sortKey="El Sayed, Khalid A" sort="El Sayed, Khalid A" uniqKey="El Sayed K" first="Khalid A." last="El Sayed">Khalid A. El Sayed</name>
<name sortKey="Elsayed, Heba E" sort="Elsayed, Heba E" uniqKey="Elsayed H" first="Heba E." last="Elsayed">Heba E. Elsayed</name>
<name sortKey="Haggag, Eman G" sort="Haggag, Eman G" uniqKey="Haggag E" first="Eman G." last="Haggag">Eman G. Haggag</name>
<name sortKey="Mady, Mohamed S" sort="Mady, Mohamed S" uniqKey="Mady M" first="Mohamed S." last="Mady">Mohamed S. Mady</name>
<name sortKey="Mohyeldin, Mohamed M" sort="Mohyeldin, Mohamed M" uniqKey="Mohyeldin M" first="Mohamed M." last="Mohyeldin">Mohamed M. Mohyeldin</name>
<name sortKey="Soliman, Randa F" sort="Soliman, Randa F" uniqKey="Soliman R" first="Randa F." last="Soliman">Randa F. Soliman</name>
</noCountry>
<country name="Royaume-Uni"><noRegion><name sortKey="Houssen, Wael E" sort="Houssen, Wael E" uniqKey="Houssen W" first="Wael E." last="Houssen">Wael E. Houssen</name>
</noRegion>
</country>
<country name="Égypte"><noRegion><name sortKey="Houssen, Wael E" sort="Houssen, Wael E" uniqKey="Houssen W" first="Wael E." last="Houssen">Wael E. Houssen</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001711 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001711 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:4703546
   |texte=   The indole alkaloid meleagrin, from the olive tree endophytic fungus Penicillium chrysogenum, as a novel lead for the control of c-Met-dependent breast cancer proliferation, migration and invasion
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26692349" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a LymphedemaV1

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

Serveur d'exploration sur le lymphœdème

The indole alkaloid meleagrin, from the olive tree endophytic fungus Penicillium chrysogenum, as a novel lead for the control of c-Met-dependent breast cancer proliferation, migration and invasion

The indole alkaloid meleagrin, from the olive tree endophytic fungus Penicillium chrysogenum, as a novel lead for the control of c-Met-dependent breast cancer proliferation, migration and invasion

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.